THP-1细胞

THP-1/THP-1细胞系/THP-1细胞株/THP-1人急性单核巨噬细胞白血病细胞

Cell line name THP-1

Synonyms THP1; THP 1; THPI; THP-1(ATCC); THP-1-O; O-THP-1; Tohoku Hospital Pediatrics-1

Accession CVCL_0006

Resource Identification Initiative To cite this cell line use: THP-1 (RRID:CVCL_0006)

Comments Group: Space-flown cell line (cellonaut).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: LL-100 blood cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: RAS genetic alteration cell panel (ATCC TCP-1031).

Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).

Population: Japanese.

Biotechnology: Used in the human Cell Line Activation Test (h-CLAT), an in vitro skin sensitization test (PubMed=16311011; PubMed=16337770; PubMed=29901211).

Characteristics: Can differentiate from monocytes into macrophage-like cells upon stimulation with PMA.

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 60-70 hours (PubMed=6970727); 26 hours (PubMed=25984343); ~35-50 hours (DSMZ=ACC-16).

Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526; Sanger).

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep promoter analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Glycoproteome analysis by proteomics.

Omics: HLA class I peptidome analysis by proteomics.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Anecdotal: Have been flown in space on Cosmos-2044 to study the inhibition of phorbol ester-induced cell activation in microgravity (PubMed=1915666).

Caution: NRAS mutation indicated incorrectly as being p.Gly12Ser in PubMed=9379676.

Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.

PubMed=1915666; DOI=10.1016/0014-4827(91)90482-A

Limouse M., Manie S., Konstantinova I., Ferrua B., Schaffar L.

Inhibition of phorbol ester-induced cell activation in microgravity.

Exp. Cell Res. 197:82-86(1991)

 

PubMed=1571549; DOI=10.1182/blood.V79.9.2378.2378

Sugimoto K., Toyoshima H., Sakai R., Miyagawa K., Hagiwara K., Ishikawa F., Takaku F., Yazaki Y., Hirai H.

Frequent mutations in the p53 gene in human myeloid leukemia cell lines.

Blood 79:2378-2383(1992)

 

PubMed=8558913

Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.

Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.

Leukemia 10:102-105(1996)

 

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D

Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Screening the p53 status of human cell lines using a yeast functional assay.

Mol. Carcinog. 19:243-253(1997)

 

PubMed=9379676; DOI=10.1016/S0145-2126(97)00036-2

Sheng X.-M., Kawamura M., Ohnishi H., Ida K., Hanada R., Kojima S., Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.

Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia.

Leuk. Res. 21:697-701(1997)

 

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588

Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.

Frameshift mutations of the hMSH6 gene in human leukemia cell lines.

Jpn. J. Cancer Res. 89:33-39(1998)

 

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886

Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Jpn. J. Cancer Res. 89:712-718(1998)

 

PubMed=10223614; DOI=10.1620/tjem.186.99

Tominaga T., Suzuki M., Saeki H., Matsuno S., Tachibana T., Kudo T.

Establishment of an activated macrophage cell line, A-THP-1, and its properties.

Tohoku J. Exp. Med. 186:99-119(1998)

 

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.

Leuk. Res. 24:255-262(2000)

 

PubMed=11066077; DOI=10.1002/1098-2264(2000)9999:9999<::AID-GCC1040>3.0.CO;2-Z

Odero M.D., Zeleznik-Le N.J., Chinwalla V., Rowley J.D.

Cytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation.

Genes Chromosomes Cancer 29:333-338(2000)

 

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

 

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.

Leuk. Res. 25:275-278(2001)

 

PubMed=11414198; DOI=10.1007/s004320000207

Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.-J.

Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

J. Cancer Res. Clin. Oncol. 127:375-386(2001)