VERO E6细胞

vero E6、vero E6、vero E6细胞、vero E6细胞、vero E6非洲绿猴肾细胞

Cell line name Vero C1008

Synonyms VERO C1008; VeroC1008; VEROC1008; VERO C 1008; VERO C1008 (E6); Vero 76 clone E6; Vero 76 clone E-6; Vero E-6; Vero E6; VERO E6; Vero-E6; VeroE6

Accession CVCL_0574

Resource Identification Initiative To cite this cell line use: Vero C1008 (RRID:CVCL_0574)

Comments Group: Non-human primate cell line.

Group: Vaccine production cell line.

Virology: Susceptible to infection by many viruses. Supports the growth of slowly replicating viruses (ATCC=CRL-1586).

Virology: Susceptible to infection by SARS coronavirus (SARS-CoV). Produces a lytic infection (PubMed=15731278; PubMed=16494729).

Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=32020029; PubMed=32511316; PubMed=33389257; PubMed=34339474).

Doubling time: 22 hours (ATCC=CRL-1586).

Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.

Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) (NCBI Taxonomy: 60711)

Hierarchy Parent: CVCL_0603 (Vero 76)

Children:

CVCL_D7CA (Abeomics Vero ACE2) CVCL_D7CB (Abeomics Vero GFP) CVCL_JX48 (sVero p66)

CVCL_E1F0 (Ubigene Vero E6 ATG7 KO) CVCL_E1F1 (Ubigene Vero E6 GSDME KO) CVCL_E1F2 (Ubigene Vero E6 PIK3C3 KO)

CVCL_E1F4 (Ubigene Vero E6 STAT1 KO) CVCL_E1F6 (Ubigene Vero E6 ZDHHC20 KO) CVCL_A7UJ (Vero E6-high ACE2)

CVCL_XD71 (Vero E6-S) CVCL_C7NK (Vero E6-TMPRSS2-T2A-ACE2) CVCL_YZ66 (Vero E6/NPC1-KO cl.19)

CVCL_C4RQ (VeroE6-Pgp-KO) CVCL_YQ49 (VeroE6/TMPRSS2)

Sex of cell Female

Age at sampling Adult

Category Spontaneously immortalized cell line

Publications

CLPUB00328

Earley E.M., Johnson K.M.

The lineage of Vero, Vero 76 and its clone C1008 in the United States.

(In book chapter) Vero cells: origin, properties and biomedical applications; Simizu B., Terasima T. (eds.); pp.26-29; Department of Microbiology, School of Medicine, Chiba University; Chiba; Japan (1988)

 

PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005; PMCID=PMC1075706

Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.

Exogenous ACE2 expression allows refractory cell lines to supportplication.

J. Virol. 79:3846-3850(2005)

 

PubMed=16494729; DOI=10.3201/eid1201.050496; PMCID=PMC3291385

Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.

SARS-associated coronavirus replication in cell lines.

Emerg. Infect. Dis. 12:128-133(2006)

 

PubMed=19016439; DOI=10.1002/9780471729259.mca04es11; PMCID=PMC2657228

Ammerman N.C., Beier-Sexton M., Azad A.F.

Growth and maintenance of Vero cell lines.

Curr. Protoc. Microbiol. 11:A.4E.1-A.4E.7(2008)

 

PubMed=32020029; DOI=10.1038/s41422-020-0282-0; PMCID=PMC7054408

Wang M.-L., Cao R.-Y., Zhang L.-K., Yang X.-L., Liu J., Xu M.-Y., Shi Z.-L., Hu Z.-H., Zhong W., Xiao G.-F.

Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro.

Cell Res. 30:269-271(2020)

 

PubMed=32511316; DOI=10.1101/2020.03.02.972935; PMCID=PMC7239045

Harcourt J.L., Tamin A., Lu X.-Y., Kamili S., Kumar-Sakthivel S., Murray J., Queen K., Tao Y., Paden C.R., Zhang J., Li Y., Uehara A., Wang H.-B., Goldsmith C., Bullock H.A., Wang L.-J., Whitaker B., Lynch B., Gautam R., Schindewolf C., Lokugamage K.G., Scharton D., Plante J.A., Mirchandani D., Widen S.G., Narayanan K., Makino S., Ksiazek T.G., Plante K.S., Weaver S.C., Lindstrom S., Tong S.-X., Menachery V.D., Thornburg N.J.

Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient.

bioRxiv 2020:972935-972935(2020)

 

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494

Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.

Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.

Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

 

PubMed=34339474; DOI=10.1371/journal.pone.0255622; PMCID=PMC8328321

Pommerenke C., Rand U., Uphoff C.C., Nagel S., Zaborski M., Hauer V., Kaufmann M., Meyer C., Denkmann S.A., Riese P., Eschke K., Kim Y., Safranko Z.M., Kurolt I.-C., Markotic A., Cicin-Sain L., Steenpass L.

Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies.

PLoS ONE 16:E0255622-E0255622(2021)