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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温,12个月
- 库存:
现货
- 供应商:
鹿森生物
- 规格:
500ml
注意事项:主要由乙醇、冰乙酸组成。
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文献和实验for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor. Resuspend the cell pellets in old media and transfer to two bottles, centrifuge as before, and decant the media. The cell pellets can be frozen at -70degC at this point. 3. Resuspend
。过滤、冷却后加Karasson-Schwh磷酸缓冲液(NaH2 PO4 ・H2 O3.31g,Na2 HPO4 ・7H2 0 33.77g,加双蒸水至1000m1)。该固定液可用于光镜、电镜免疫细胞化学观察。固定时间以6-18小时为宜。 (3)AAF液:为较常用的固定液(95%一100%乙醇85ml,冰醋酸5ml,浓甲醛l0ml)。 (4)Clarke改良固定剂(100%乙醇75ml,冰醋酸25m1),常用于冰冻切片的后固定。
Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation
under the same conditions. Then, the 53 ml of the culture was divided into 4X 2 liter flasks containing 1 liter of the same medium and incubated for additional 8-10 hours. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 500 ml bottle in the RC5-B
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