- $216
- LSM BIO
- 进口
- PVT83674
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pLV3-CMV-3×FLAG-YY1-del(ATG)-Puro Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pLV3-CMV-3×FLAG-YY1-del(ATG)-Puro Plasmid
Catalog No.: PVT83674
pLV3-CMV-3×FLAG-YY1-del(ATG)-Puro Plasmid
Alias:NM_003403.5;DELTA; GADEVS; INO80S; NF-E1; UCRBP;YIN-YANG-1
Host: Mammalian cells,lentivirus
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling:
Promoter: CMV
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验How to insert one single nucleotide into a plasmid-Mole
----------------------- QUOTE(Kersten @ Dec 12 2005, 07:03 AM) [snapback]34264[/snapback] in general if expressed in e.coli the first ATG next to the ribosomal binding site should be were translation starts (mostly) I mean in the mammalian cells. such as CMV promoter
translation. An upstream enhancer/promoter aids expression. The cloned gene insert must provide the translational start site ATG. Also in this plasmid are genes encoding neomycin phosphotransferase, which gives protection to transfected cells from the G-418
in eukaryotic cells. The MCS in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP
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