- $216
- LSM BIO
- 进口
- PVT82899
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pT3-EF1a-Myc-3×HA-IRES-luc Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pT3-EF1a-Myc-3×HA-IRES-luc Plasmid
Catalog No.: PVT82899
pT3-EF1a-Myc-3×HA-IRES-luc Plasmid
Alias:NM_010849.4;bHLHe39; Myc2; Niard; Nird
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers:
Fluorescence labeling: Fluc
Promoter: EF1a
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验) ，Neo(G418)，Zeo，Hygro，其中Puro和BSR是较常用的真核抗性。真核抗性常用于稳定株筛选等过程，具体使用抗性筛选的浓度可通过空细胞抗性预实验确认，浓度梯度可根据抗生素说明书推荐浓度范围设置。(4)蛋白标签：蛋白标签（protein tag）：与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。常见标签：3xFLAG，6xHis，HA，Myc ，OLLAS，GST等。需注意目的蛋白是否有功能肽如信号肽，前肽等，蛋白标签需要融合无功能肽的一端
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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