- $216
- LSM BIO
- 进口
- PVT82135
- 2025年07月11日
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- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pT7-6×His-GFP(1-10) Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pT7-6×His-GFP(1-10) Plasmid
Catalog No.: PVT82135
pT7-6×His-GFP(1-10) Plasmid
Alias:-
Host: E.coli
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Empty vetor
Prokaryotic resistance: Kan
Screening Markers:
Fluorescence labeling: Green
Promoter: T7
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒
♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒
, and resuspend in 10 µl of TE, pH 8.0. Use 2 µl of each for analysis on a 1% agarose gel. Purification of cosmids and low-copy-number plasmids From: QIAGEN Plasmid Mini Handbook Cosmids and low-copy-number plasmids often require large culture volumes
for ca. 10 min on ice . 8.spin for 10 min. 9.remove 400 ml supernatant and transfer to new tube prefilled with 400 ml isopropanole. 10.vortex well and keep tube on ice for 10 min . 11.spin for 10 min . 12.wash
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