- $216
- LSM BIO
- 进口
- PVT80848
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pAAV-EF1a-TurBoID-Armc12(2-341aa) Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pAAV-EF1a-TurBoID-Armc12(2-341aa) Plasmid
Catalog No.: PVT80848
pAAV-EF1a-TurBoID-Armc12(2-341aa) Plasmid
Alias:NM_026290.3;4930511I11Rik
Host: Mammalian cells,Adeno associated virus
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers:
Fluorescence labeling:
Promoter: EF1a
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
. 2) For point mutation - 12 cycles, single aa change - 16 cycles, multiple aa deletions or insertions - 18 cycles. 3) Be careful to avoid carrying over mineral oil when transforming cells - it can seriously reduce transformation efficiency. 4) If you fail
Transfection of Mammalian Cells Using Lipofectamine
at 37C in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. 3. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 µg DNA (plasmid) in 375 µl serum-free
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