- $260
- LSM BIO
- 进口
- PVT79540
- 2026年03月06日
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- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-EGFR-L858R-T790M-ECFP-Neo Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pCMV-EGFR-L858R-T790M-ECFP-Neo Plasmid
Catalog No.: PVT79540
pCMV-EGFR-L858R-T790M-ECFP-Neo Plasmid
Alias:NM_005228.5;ERBB;ERBB1;HER1;mENA;NISBD2;PIG61;
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Kan
Screening Markers: Neo/G418
Fluorescence labeling: cyan
Promoter: CMV
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验。在中国 NSCLC 患者中，EGFR 突变率约为 30％，其中腺癌患者的突变率高达 50％ 左右。研究发现，EGFR-T790M 突变小鼠以及 EGFR-L858R-T790M 突变小鼠比 EGFR-L858R 单突变小鼠的肿瘤潜伏期要更长，更适适用于耐药性机制研究[4]。 图 3. EGFR 相关信号通路 集萃药康针对 EGFR 突变靶点也做了相应的布局，包括 20 号外显子 T790M 点突变，C797S 突变及 21 号外显子 L858R 突变小鼠模型，与组织特异性 cre 鼠配繁
, and resuspend in 10 µl of TE, pH 8.0. Use 2 µl of each for analysis on a 1% agarose gel. Purification of cosmids and low-copy-number plasmids From: QIAGEN Plasmid Mini Handbook Cosmids and low-copy-number plasmids often require large culture volumes
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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