- $260
- LSM BIO
- 进口
- PVT77914
- 2025年12月20日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-EGFP-POLG-Del(1401C)-FLAG-HA-Neo Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pCMV-EGFP-POLG-Del(1401C)-FLAG-HA-Neo Plasmid
Catalog No.: PVT77914
pCMV-EGFP-POLG-Del(1401C)-FLAG-HA-Neo Plasmid
Alias:-
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Amp
Screening Markers: Neo/G418
Fluorescence labeling: Green
Promoter: CMV
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
Gene gun administration of DNA is an invaluable technique for transfecting tissues with only 1 μg DNA/shot. Here, we describe a transfection technique of healthy corneas of BALB/c mice with a standard gene gun, using a technique
of Solution II. Mix, incubate on ice for 5 min. 5) Add 300 µL of Solution III. Mix, incubate on ice for 5 min. 6) Spin 5 min at maximum speed, 4°C. Save supernatant in a fresh tube. 7) Add 10 µL of RNAseA, incubate at 37°C x5 min. 8) Extract
Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C, 230-250rpm (best in 5ml bijou) Spin yeast culture at 13,000rpm for 5 min (microfuge) Pour
技术资料暂无技术资料 索取技术资料
