- $216
- LSM BIO
- 进口
- PVT76995
- 2025年10月18日
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- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pPSU150 Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pPSU150 Plasmid
Catalog No.: PVT76995
pPSU150 Plasmid
Alias:Plasmid꜌
Host: E.coli
Use(s): Protein expression
Fragment Type: ORF
Fragmented species:
Prokaryotic resistance: Amp
Screening Markers:
Fluorescence labeling:
Promoter: T7
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验qisanni05 请教各位大侠，为什么我在MiRbase上找不到hsa-mir-150 基因上下游序列呢，请各位高手帮忙找一下，十分感谢！ qisanni05 这是什么意思啊，由于刚涉及这方面还不太懂，还请高手多指教，非常感谢！ lide658 你是要找编码hsa-mir-150的序列吗？ 如果是想构建hsa-mir-150表达载体，在MiRbase上是找不到的，你只能
Production of Heterologous Proteins in Yeast With the Aid of the Hsp150 Carrier
glycoproteins can be produced in Saccharomyces cerevisiae and Pichia pastoris by joining them to a fragment of a natural secretory glycoprotein of S. cerevisiae , Hsp150Δ. The performance of the Hsp150Δ carrier in both yeasts appears to exceed that of the MFα
for 30 sec . 3.decant supernatant and resuspend pellet in 100 ml lysis solution . 4.add 200 ml alkaline SDS, vortex well . 5.incubate for 5 min on ice . 6.add 150 ml high salt solution, vortex well. 7.incubate
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