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- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pGAG-MS2 Plasmid
- 库存:
100
- 供应商:
LSM BIO
- 规格:
2ug
pGAG-MS2 Plasmid
Catalog No.: PVT77182
pGAG-MS2 Plasmid
Alias:-
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Virus
Prokaryotic resistance: Amp
Screening Markers:
Fluorescence labeling:
Promoter: CAG
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport During Drosophila Oogenesis
to visualising mRNA localisation in vivo, in which RNAs containing MS2 binding sites are labelled by the MS2 coat protein fused to fluorescent reporters. We discuss the use of this mRNA-MS2/MS2CP-FP system to study mRNA localisation during Drosophila oogenesis
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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