- $216
- LSM BIO
- 进口
- PVT75893
- 2025年08月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
LZRS ERTm RasV12-HA Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
LZRS ERTm RasV12-HA Plasmid
Catalog No.: PVT75893
LZRS ERTm RasV12-HA Plasmid
Alias:·
Host: Mammalian cells,retrovirus
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human,Mouse
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling:
Promoter:
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验In vitro transcription with yeast nuclear extract
buffer 500 mM Potassium Acetate, pH 7.6100 mM HEPES, pH 7.6 5 mM EDTA 25 mM MgOAc Creatine phospho kinase (sigma C-3755 165 units/mg) 2.0 mg/ml in HA + 0.1 M potassium acetate Stored in 12 microliter aliquots at -70 degrees. The enzyme is stable
In vitro transcription with yeast nuclear extract
in HA + 0.1 M potassium acetate Stored in 12 microliter aliquots at -70 degrees. The enzyme is stable to a few freeze thaw cycles if thawed on ice and quickly refrozen. Phospho creatine (64 mg/ml) 128 mg phospho creatine 2 ml TE pH
insertion. The popout event is mediated by cre recombinase and requires induction of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA tag can be detected by mouse monoclonal antibodies 12CA
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