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- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pEX18Tc-FlhA(PAO1) Plasmid
- 库存:
100
- 供应商:
LSM BIO
- 规格:
2ug
pEX18Tc-FlhA(PAO1) Plasmid
Catalog No.: PVT75878
pEX18Tc-FlhA(PAO1) Plasmid
Alias:PWU33336.1
Host: E.coli
Use(s): gene edit
Fragment Type:
Fragmented species:
Prokaryotic resistance: Tet
Screening Markers: SacB
Fluorescence labeling:
Promoter:
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验in the design of targeting vectors and the subsequent functional analysis of newly discovered genes. Manipulation of plasmid DNA by homologous recombination was first reported in yeast (9) and since then techniques have been developed to include phage
(mRNA or DNA) and a transposon plasmid containing IR/DR sequences that flank any cargo DNA of interest. Once expressed, the transposase recognizes and binds the IR/DRs and catalyzes the precise integration into a small TA dinucleotide target site
ASTRACT We describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types. A plasmid carrying green fluorescent protein (GFP) is co-transfected
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