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| 重组nCoV-N蛋白(lambda) | HP-N011 | 100μg |
| 500μg | ||
| 1mg |
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文献和实验debris. Transfer the supernatant to a fresh tube. 17x100mm disposable polypropylene tubes (Falcon brand has pop-top cap) are convenient. These can be rinsed and re-used. 6.Add DNase I (final is 1μg/ml) and RNase AI (final is 10μg/ml). For stocks
Recombineering/Lambda red-mediated gene replacement
culture to 30 mL LB medium (1:100 dilution) Check culture density every 30 minutes starting at +1 hour; grow to OD600 of 0.3 to 0.4 OD600 measurements of K91 +2:00 hrs: 0.06 +2:45 hrs: 0.3008
Purification of DNA from lambda phage
This is a modification of the procedure in Short Protocols (1-41,1-45) 1.Prepare a 50 ml liquid lysate: A.mix 2xlO8 E.coli cells with 100μl phage (from one picked plaque in 500μl SM),and 100μl lOmM MgCl2/lOmM CaCI2.Adsorb at 37℃ for 15 min. B
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