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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Brigham & Women's Hospital, Boston, MA 02115 Excerpted from Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion
Purification of Baculovirus-Expressed Human DNA Topoisomerase I
for htopo I constructs fused to either the T7 gene 10 translation initiation signal (6 ) or to glutathione-S-transferase (GST). Furthermore, expression in protease-deficient strains of E. coli does not appreciably reduce the proteolytic breakdown of htopo
receptors or their domains in insect cells (6 ), Escherichia coli (7 ), or mammalian cells (8 ), has facilitated their purification. Purification with histidine (His)-tagged proteins is advantageous, because, unlike protein tags, such as glutathione S
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