Recombinant Human Chordin-like

Purification of recombinant sBRF M166L

under lac iQ control Kanomycin resistant) Inoculate 6 ml of a saturated overnight culture of the transformed bacteria into 2X 1 Liter of YT Amp (50 ug/ml) (and Kan (30 ug/ml) if using strain JM25) (adding 1-2 drops of antifoam A before autoclaving the YT

Generation of Recombinant Herpes Simplex Virus Ampiicons

capsids ( 1 – 4 ). As shown in Fig. 1 , amplicon vector can be amplified into head-to-tail concatemers and then packaged into defective HSV-1 viral particles up to one genome size (~150 kb) in the presence of HSV-1 helper viruses

Purification of Human Multiprotein Complexes using OneSTrEP Technology

  Introduction Here we describe a strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed