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文献和实验Intracellular Cytokine Staining Protocol
stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24 hours stimulation
Neutralizing Bioassay Protocols
L929 1 10 600 24 MP1-22E9 GM-CSF MC/9 1 10 1250 48 Table 2: Human Neutralization Assay Quick Guide Chart
Intracellular Cytokine Staining Protocol
. Note: Optimal stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24
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