- 询价
- Agrisera
- Sweden
- AS20 4443-DL650
- 2026年07月12日
- Immunofluorescence (IF), Western blot (WB)
- Rabbit
- Recombinant GFP overexpressed in E,coli, from: Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212
- 形态：
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
- 保存条件：
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
- 克隆性：
Polyclonal
- 适应物种：
Recombinant GFP overexpressed in E,coli, from: Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum
- 供应商：
Agrisera
- 宿主：
Rabbit
- 应用范围：
Immunofluorescence (IF), Western blot (WB)
- 靶点：
Anti-GFP | Green Fluorescence Protein - DyLight® 650 conjugated (40 µg)
- 抗体英文名：
Anti-GFP | Green Fluorescence Protein - DyLight® 650 conjugated (40 µg)
- 抗体名：
Anti-GFP | Green Fluorescence Protein - DyLight® 650 conjugated (40 µg)
- 规格：
40 µg
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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
) and allow it to stand at room temperature fo 30-45 minutes (Sol. A) 3) For each transfection, dilute 50 µg GFP DNA in 100µl of OptiMem® (Life Technologies) serum-free medium in a sterile microfuge tube (Solution B). 4) Combine Solutions A and B
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
Introduction Green fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien, 1996; Tsien, 1998). The validation and interpretation of these data
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
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