- 询价
- Agrisera
- Sweden
- AS22 4758
- 2026年01月10日
- ELISA (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
- Mouse
- MBP maltose binding protein epitope tag
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
MBP epitope tag protein.
- 亚型：
IgG1/ Kappa
- 形态：
Purified on Protein A to a total immunoglobulin fraction, in Tris 0,1M, glycine 0,1M and 2 % sucrose.
- 保存条件：
Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
- 克隆性：
Monoclonal
- 适应物种：
MBP maltose binding protein epitope tag
- 供应商：
Agrisera
- 宿主：
Mouse
- 应用范围：
ELISA (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
- 靶点：
Anti-MBP | Maltose-binding protein (monoclonal, clone 16D3)
- 抗体英文名：
Anti-MBP | Maltose-binding protein (monoclonal, clone 16D3)
- 抗体名：
Anti-MBP | Maltose-binding protein (monoclonal, clone 16D3)
- 规格：
100 µg
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文献和实验Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
Maltose-Binding Protein as a Solubility Enhancer
as a general solubilizing agent, this has not turned out to be the case. In a direct comparison with glutathione S-transferase (GST) and thioredoxin, maltose-binding protein (MBP) was decidedly superior at solubilizing a diverse collection of aggregation-prone
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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