- 询价
- Agrisera
- Sweden
- AS21 4678
- 2026年07月08日
- ELISA (ELISA), Western blot (WB)
- Mouse
- MBP maltose binding protein epitope tag
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
MBP epitope tag recombinant protein
- 形态：
Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.
- 保存条件：
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- 克隆性：
Monoclonal
- 适应物种：
MBP maltose binding protein epitope tag
- 供应商：
Agrisera
- 宿主：
Mouse
- 应用范围：
ELISA (ELISA), Western blot (WB)
- 靶点：
Anti-MBP | Maltose Binding Protein (monoclonal)
- 抗体英文名：
Anti-MBP | Maltose Binding Protein (monoclonal)
- 抗体名：
Anti-MBP | Maltose Binding Protein (monoclonal)
- 规格：
50 µg
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文献和实验Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
Maltose-Binding Protein as a Solubility Enhancer
as a general solubilizing agent, this has not turned out to be the case. In a direct comparison with glutathione S-transferase (GST) and thioredoxin, maltose-binding protein (MBP) was decidedly superior at solubilizing a diverse collection of aggregation-prone
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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