
Anti-Tubulin alpha chain, Biot
in conjugated (clone TU-01)相关产品推荐更多 >

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- 文献和实验
- 技术资料
- 免疫原:
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. UniProt: Q71U36
- 亚型:
IgG1
- 形态:
Conjugated with biotin-LC-NHS under optimum conditions and free of unconjugated biotin
- 保存条件:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- 克隆性:
Monoclonal
- 适应物种:
Arabidopsis thaliana, Eisenia, Nicotiana tabacum, Paramecium, Saccharomyces cerevisiae, human, mouse, pig, turkey
- 供应商:
Agrisera
- 宿主:
Mouse
- 应用范围:
Immunohistochemistry (IHC) paraffin, Immunocytochemistry (ICC), Flow cytometry (Flowcyt), Western blot (WB)
- 靶点:
Anti-Tubulin alpha chain, Biotin conjugated (clone TU-01)
- 抗体英文名:
Anti-Tubulin alpha chain, Biotin conjugated (clone TU-01)
- 抗体名:
Anti-Tubulin alpha chain, Biotin conjugated (clone TU-01)
- 规格:
0.1 mg
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
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