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Anti-GFP | Green Fluorescence

Protein, clone 1A5 (rat monoclonal)
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  • 询价
  • Agrisera
  • Sweden
  • AS20 4511
  • 2026年01月06日
  • Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
  • Rat
  • Native GFP, Recombinant GFP (E. coli), all variants of GFP, including EGFP
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant GFP protein from Aequorea victoria, UniProt: P42212

    • 亚型

      IgG1

    • 形态

      Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.

    • 保存条件

      Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

    • 克隆性

      Monoclonal

    • 适应物种

      Native GFP, Recombinant GFP (E. coli), all variants of GFP, including EGFP

    • 供应商

      Agrisera

    • 宿主

      Rat

    • 应用范围

      Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

    • 靶点

      Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

    • 抗体英文名

      Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

    • 抗体名

      Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

    • 规格

      100 µg

    GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

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    图标文献和实验
    该产品被引用文献
    Maehara et al. (2015). issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP)Okazaki et al. (2012). Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33(10):1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)
    相关实验
    • Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins

      of the jellyfish, Aequoria victoria (1 , 2 ). The original expression plasmid for this jellyfish protein, green fluorescent protein (GFP) yielded a low level of fluorescence. However, through mutagenesis, enhanced GFPs have been produced that are about 35 times

    • Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enzymes

      The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria is a member of a small but important class of proteins that exhibit strong visible fluorescence without the requirement of cofactors or other enzymes

    • 【共享】如何检测GFPGFP、YFP、EYFP、CFP抗体?

      检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体GFP是绿色萤光蛋白(Green Fluorescent Protein)的简称,由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质,在蓝色波长范围的光线激发下,会发出绿色萤光。GFP或其突变体EGFP标签系统等被广泛用于基因表达效率的检测,以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。我们都知道GFP抗体能应用于GFP的表达检测、纯化

    图标技术资料

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    文献支持
    Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)
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