3-Bromo-5,8-dichloro-4-hydroxy

P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants

expressing the reporter gene within the cellular context of the explant or tissue. A qualitative hrstochemical assay to measure GUS activity is based on the substrate 5-bromo-4-chloro-3-mdolyLl3-o-glucuronide (X-Glut) ( 1 ). The GUS enzyme hydrolyzes X-Glut

Synthesis of (2S, 4S, 5S)-5-(t-Butoxycarbonylamino)-4-(t-Butyldimethylsilyloxy)-2-Isopropyl-7-Methyl Octanoic Acid

to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N

Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry

with fluorochromes, defined as the TUNEL assay. This chapter describes the variant based on DSBs labeling using 5-Bromo-2′-deoxyuridine-5′-triphosphate (BrdUTP) as a TdT substrate and the incorporated BrdU is subsequently detected immunocytochemically with anti