Recombinant GM-CSF (Granulocyt

gene_symbol
Description：Recombinant GM-CSF (Granulocyte-macrophage colony-stimulating factor)，Human
Accn
Unipro ID：P04141
Synonyms：colony stimulating factor 2, CSF2, CSF
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE with polyhistidine tag at the N-terminus.
Tags：His Tag (N-term)
PredictedMW：The protein has a calculated MW of 15.4 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Stability
Bioactivity：Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is 1 x 10⁷ IU/mg.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.