Recombinant IL-10 (Interleukin

gene_symbol
Description：Recombinant IL-10 (Interleukin-10)，Human
Accn
Unipro ID：P22301
Synonyms：B-TCGF, CSIF, TGIF
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN with polyhistidine tag at the C-terminus
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 19.6 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Stability
Bioactivity：Measure by its ability to induce MC/9‑2 cells proliferation. The ED₅₀ for this effect is 1x10⁶ IU/ mg.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.01 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.