Recombinant BMP-4 (Bone morpho

gene_symbol
Description：Recombinant BMP-4 (Bone morphogenetic protein-4)，Human
Accn
Unipro ID：P12644
Synonyms：BMP-2B, DVR4
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR with polyhistidine tag at the C-terminus.
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 12.88 kDa. The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium carbonate, pH 9.0.
Stability
Bioactivity：Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.58 ng/mL.
Purity：>95% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Bone Morphogenetic Protein-4 (BMP-4) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-4 can bind with the TGF-β receptor and trigger SMAD protein signal transduction. BMP-4 has a significant expression in the prostate, it plays a vital role in the development of mesoderm into bones and the development of the epidermis and Dorsal-Ventral axis.