VEGF-A (Isoform 165) human rec

gene_symbol：VEGFA
Description：VEGF-A (Isoform 165) human recombinant protein, 20 µg
Accn：NM_001025366
Unipro ID：P15692
Synonyms：VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Species：Human
Amount：20 ug
Delivery time：7周
Expression sequence：APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Tags
PredictedMW：45 kDa
Buffer：Presentation State: PurifiedState: Lyophilized purified protein.Buffer System: 50 mM Acetic Acid without stabilizer.
Stability：Shelf life of the lyophilized product at -20°C:  one year from despatch.
Bioactivity：Biological: The ED50 for stimulation of cell proliferation by Human umbilical vein endothelial  (HUVEC) cells has been determined to be in the range of 1-4 ng/ml.
Purity：>95% pure by SDS-PAGE.
Concentration
Preparation：Lyophilized purified protein.
Endotoxin：< 1 EU/µg.
Shipping
Background：This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020]