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- OriGene
- TP790051
- 2025年11月08日
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Description：Purified fluorescent protein mKate, with N-terminal HIS tag, expressed in E. coli, 250ug
Accn：AEX25288
Unipro ID
Synonyms
Species：Escherichia coli
Amount：250 ug
Delivery time：现货
Expression sequence：MVSELIKENM HMKLYMEGTV NNHHFKCTSE GEGKPYEGTQ TMRIKAVEGG PLPFAFDILA TSFMYGSKTF INHTQGIPDF FKQSFPEGFT WERVTTYEDG GVLTATQDTS LQDGCLIYNV KIRGVNFPSN GPVMQKKTLG WEASTETLYP ADGGLEGRAD MALKLVGGGH LICNLKTTYR SKKPAKNLKM PGVYYVDRRL ERIKEADKET YVEQHEVAVA RYCDLPSKLG HRGGGGSGLR
Tags：N-His
PredictedMW：26.5 kDa
Buffer：PBS, pH 7.4, 10% glycerol
Stability：Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity：> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration：>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
Shipping
Background
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文献和实验Strategies to Optimize Protein Expression in E. coli
, N., and Sharma, A. 2002. Extra terminal residues have a profound effect on the folding and solubility of a Plasmodium falciparum sexual stage‐specific protein over‐expressed in Escherichia coli. Eur. J. Biochem. 269:5259‐5263
Site‐Specific Protein Labeling with SNAP‐Tags
Srikun, D., Albers, A.E., Nam, C.I., Iavarone, A.T., and Chang, C.J. 2010. Organelle‐targetable fluorescent probes for imaging hydrogen peroxide in living cells via SNAP‐Tag protein labeling. J. Am. Chem. Soc. 132:4455–4465
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
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