- ￥1999
- null
- TA424411
- 2025年07月09日
- Rabbit
- null
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. Store at -24°C long term. Avoid freeze / thaw cycle.
- 克隆性：
Monoclonal
- 适应物种：
null
- 宿主：
Rabbit
- 规格：
100 ul
description：Phospho-Nucleolin (T84) Antibody
symbol：NCL
synonyms：NCL; Nucl; Nucleolin; Protein C23
locusid：4691
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：P19338
Applications：IHC, WB
CNdelivery：2周
RecommendDilution：WB 1:500~1:2000 IHC 1:50~1:200
Reactivity：Human
Immunogen：A synthesized peptide derived from human Phospho-Nucleolin (T84)
Purification：Affinity-chromatography
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：nucleolin
Background：Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles.
Buffer：Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter：IgG
predicted_size：100kDa
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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking
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