- ￥1999
- null
- TA422208
- 2025年07月09日
- Rabbit
- null
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. Store at -23°C long term. Avoid freeze / thaw cycle.
- 克隆性：
Monoclonal
- 适应物种：
null
- 宿主：
Rabbit
- 规格：
100 ul
description：ER alpha Antibody
symbol：ESR1
synonyms：ER; Era; ESR; ESR1; ESRA; NR3A1
locusid：2099
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：P03372
Applications：ChIP, FC, ICC/IF, IHC, WB
CNdelivery：2周
RecommendDilution：WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 ChIP 5 ?g/30 ?g FC 1:30
Reactivity：Human, Mouse, Rat, Dog
Immunogen：A synthesized peptide derived from human ER alpha
Purification：Affinity-chromatography
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：estrogen receptor 1
Background：ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.
Buffer：Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter：IgG
predicted_size：60kDa
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12... Staining for surface receptors by feeding antibody (Von Zastrow lab) This protocols allows the use of fluorescence microscopy to visualize epitope
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
is described below. The epitope retrieval protocol is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. Some Zymed® antibodies may require epitope retrieval protocol (e.g., estrogen receptor
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