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- 禾午生物
- P59295
- 国产
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pCMV-Ubiquitin(human)-K48-3×Myc-Neo
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃&负80℃
- 规格：
干粉&甘油菌
| 名称 | pCMV-Ubiquitin(human)-K48-3×Myc-Neo |
| 基因长 | 228bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 人 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pCMV-MCS-3×Myc-Neo |
| 正向引物 | CMV-F |
| pCMV-EGFP-RAB1B(human)-Neo |
| pCMV-EGFP-RAB2A(human)-Neo |
| pCMV-EGFP-RAB2B(human)-Neo |
| pCMV-HSPA9(human)-3×FLAG-EGFP-Neo |
| pCMV-EGFP-PCDHB2(human)-3×FLAG-Neo |
| pCMV-Dhrs3(mouse)-3×FLAG-Neo |
| pCMV-IRAK1(human)-3×Myc-Puro |
| pCMV-ATP5F1A(human)-3×Myc-Puro |
| pCAGGS-Agrn(mouse)-Myc |
| pCMV-Ubiquitin(human)-K48-3×Myc-Neo |
| pCMV-STC1(human)-EYFP-3×FLAG |
| pCMV-KLHL32(human)-3×HA-Neo |
| pCMV-Dgat2(mouse)-3×HA-Neo |
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文献和实验Several important signaling processes depend on the tagging of cellular proteins with “polyubiquitin chains”—ubiquitin polymers whose building blocks are connected by isopeptide bonds between G76 of one ubiquitin and a specific lysine residue
Measuring Ubiquitin Conjugation in Cells
Protein ubiquitination is crucial to many diverse and critical functions of cells. Although it has been long known that conjugation of ubiquitin to proteins results in their destruction by the proteasome, recently it has become apparent
Assays for RING Family Ubiquitin Ligases
Many eukaryotic proteins are regulated by the covalent attachment of ubiquitin or polyubiquitin chains. These include proteins involved in cell cycle control, tumor suppression, and many signaling pathways. Ubiquitination of proteins occurs
