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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
p32 Inhibitor M36
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
802555-85-7
- 规格:
P654717-1ml
英文名:p32 Inhibitor M36
纯度或规格:10mM in DMSO
SPU:P654717
CAS:802555-85-7
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验MINICHROMOSOME MICROTUBULE BINDING ASSAY
5M NaCl 1mM taxol in DMSO 2X ASSAULT BUFFER: 5mL 10% SDS, UltraPure 5mL 0.5M EDTA 5mL HEPES-KOH, pH 7.6 [7.5 w/NaOH] 85mL H2 O **2X A.B. can be frozen in 10mL aliquots. [3.5mL
Minichromosome-Microtubule Binding Assay 微染色体-微管结合实验
or a factor, include the inhibitor in the H2O wash. SPHEROPLASTING. Re-suspend cells in 4mL YWB (containing 10mM beta-mercaptoethanol and 1mM PMSF). Le sit at room temperature for 2-3 min. Add 100 uL glusulase. Incubate at 23o C
微染色体-微管结合实验(Minichromosome-Microtubule Binding Assay)
1.5-2.0K for 8-10min. SPHEROPLASTING. Resuspend cells in 4mL YWB. *NOTE: If cells have been arrested with nocodazole or alpha factor, include the inhibitor in the YWB. Let sit at room temperature for 2-3 min. Add oxalyticase [12uL 1mg
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