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- 文献和实验
- 技术资料
- 库存：
期货
- 规格：
M661649-1ml
中文名：MYF-03-176
英文名：MYF-03-176
纯度：10mM in DMSO
货号：M661649
存储温度：避光,干燥,-80℃储存
运输条件：特低温运输
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文献和实验Natural History Museum Protocol for EST sequencing from lamb
- (if the agarose gel in step 4 only shows a weak band, use less than 50µl of water to elute). Direct Purification Buffer: 50mM KCl (=0.37g/100ml) 10mM Tris-HCL (pH 8.8 at 25℃) (=0.12g/100ml) 1.5mM MgCl2 (=0.03g/100ml) 0.1% Triton X-100 (=100µl/100ml
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
1 drop of chloroform and 35µl DMSO (i. e. 7% final) and place at -70oC (no need to snap freeze). (N. b. store chloroform with some anhydrous sodium bicarbonate in the bottom of the bottle, about 1 teaspoon per 100mls.) (3) PCR
transcription-PCR where cDNA is first synthesized at 55° - 70°C in the presence of manganese. The buffer contains DMSO and glycerol. One version of this enzyme (rTth DNA polymerase XL) has proofreading activity via its 3'' to 5'' exonuclease activity and is used
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