- ￥567.90
- 阿拉丁
- 上海
- N164065
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C储存;充氩
- 英文名：
NHS-PEG-NHS
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
N164065-1g
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
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文献和实验-NHS在磷酸盐缓冲液中结合2 h。反应后，在含50 mM PO4、50 mM NaCl和10%甘氨酸的磷酸缓冲液中使吡咯-链酶亲和素结合物脱盐。 小鼠IgG与吡咯-NHS的结合与上述方法相同，并用作阴性对照。 点样溶液（含10%甘油的磷酸盐缓冲液）包含20 mM的自由吡咯和浓度为12 mM、8 mM和4 mM的链酶亲和素或小鼠IgG抗体。这些生物分子通过电化学过程固定在生物芯片表面。电聚合过程中，工作电极（棱镜金表面）和对电极（点样针）间生成电脉冲（2 V，100
according to the instructions described in the Product Information Sheet provided with your microspheres.2.Transfer 5.0 x 106 of the stock microspheres to a USA Scientific microcentrifuge tube.3.Pellet the stock microspheres by microcentrifugation at ≥ 8000 x g for 1-2
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