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- F2502-2
- 2025年07月11日
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F2502-2
F2502-2赛默飞30MM NYLON + GLASS注射式过滤器, 0.2 UM, 100/包 Thermo Fisher
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文献和实验Clone Genes From a Phage Library
nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform (see the end for recipes).Ex-assist phage as well for plasmid excision
+ 0.2 percent maltose (to express the mal permease which is the lambda receptor) overnight at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4. These bacteria will be good to use for phage plating
Clone Genes From a Phage Librar
+ 0.2 percent maltose (to express the mal permease which is the lambda receptor) overnight at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4. These bacteria will be good to use for phage plating
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