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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 供应商:
天根生化科技(北京)
- 规格:
1.8ml
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文献和实验The Polymerase Chain Reaction (PCR)
• Template DNA • Reaction buffer (Tris, ammonium ions(and/or potassium ions), magnesium ions,bovine serum albumin) • Nucleotides (dNTPs) • Primers • DNA polymerase (usually Taq) PCR In Detail • Denature, anneal, extend and repeat the cycle 30 to 35
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
Mouse p27 PCR Using Gitschier Buffer
with H2O and aliquot into 1.5 mL eppendorf tubes PCR Reaction Mix (To make a fresh master mix, multiply # PCR reactions x volumes, below) 2 µL KG-1 (10x) 1x 2 µL KG-2 (10x) 1x 2 µL Primer 1 (1uM) 0.1 mM 2 µL Primer 2 (1uM) 0.1 mM
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