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文献和实验Phenylethanolamine N -methyltransferase (PNMT) (EC 2.1.1.28, S -adenosyl-L -methionine: phenylethanolamine N -methyltransferase) is the enzyme that catalyzes the biosynthesis of norepinephrine [noradrenaline; (NA)] to epinephrine [adrenaline
of endogenous DNA.By the same token,a higher cellular protein concentration will also lead to a higher negative control for similar reasons ( protein methylation). If your positive controls are too low: Did you deviate from protocol? (e.g.,not drying
, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using radioactively labeled AdoMet and biotinylated DNA substrates that is ideal for enzymatic characterization of these enzymes. (2) An assay using bisulfite
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