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- 2026年08月14日
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文献和实验45Ca2+ uptake study in PC12 cells
, 2mM CaCl2, 10 mM Hepes, pH 7.4) every 15 minutes for 1 hour at 37o C. 3. Add 45Ca2+ (2 µCi/ml) to Ca2+ -free buffer (secretion buffer without 2 mM CaCl2), and drugs to the labelled buffer at 37o C. 4. Incubate the cells with labelled
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we have found to quantitate the distribution of the oxidized product is to use the mitochondrion-selective MitoTracker Green FM stain (M7514) in conjunction with the RedoxSensor Red CC-1 stain.45 JC-1 and JC-9: Dual-Emission Potential-Sensitive Probes The green
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
before use. 2. Turn on the microscope and thermo plate to prewarm. 3. Prepare one 60-mm Petri dish containing 15-20 drops (20 µL per drop) of medium with HEPES (Ca2+ /Mg2+ -free) containing 5% SPS, one 30-mm Petri dish
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