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- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Kluyveromyces lactis RIM21 Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
pH-response regulator protein palH/RIM21Editorial/Sponsord
EditorialUniprot ID
Q6CXK7Gene Names
RIM21Organism
Kluyveromyces lactisAASequence
MTGRSRWRYHFPSEDYSSCQGVQLDEGVLIWNKLPDKFAYIKSLVFTSNCQDGTPLYSSF VELEDECPYLPIVAFDWNSYINNDGYTGPFKYSIYSVIYSVTANFIITVFLTVIVFINVP TKPSRKASYILKLGALLASLNLSIFVIRVWKNIAENYTYNGYVSSTSFLNLLWGDKVFAG IDLVVVFLLQISQVQIVMRFFDRMQEKRMVFYFGLFLILVTQILWVIPTLSPVPTKDSDS DIDILPPFVYLFRIALSTCYASVICFHVLTKKSLCFRGRMIFLTLLTIFTVFLHPTFFIV DVSNLWIDDLSEIFNTTCYLASTVIVLEWTNRIHTMERKIHAASVLGRPIYEDEEQNFHF AIYALRMQNAMKMHRDGHVHDHGHTCDSNDNANVSLQNANTNDSSSIITDNSSQRTSTFG TMATDQVTLHTQQGERNITQFKRAETFWNKCHHVLESIVYYTDNVMVNALAGSKTFDSFT NDAEKLLKTERLRKTVGLDEPEDVYLYRKTTMSSDSVMEExpression Region
1-519aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1914Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
sepharose beads (10,000 RPM for 1 minute at 4°C) and save the supernatants as unbound fraction (input for step 21 below);Wash the pelleted Protein A/Salmon sperm sepharose beads bound to the putative protein of interest twice with: 1ml ChIP lysis
/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time
质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
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