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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Vaccinia virus OPG099 Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Protein L5Editorial/Sponsord
EditorialUniprot ID
P68622Gene Names
OPG099Organism
Vaccinia virusAASequence
MENVPNVYFNPVFIEPTFKHSLLSVYKHRLIVLFEVFVVFILIYVFFRSELNMFFMPKRK IPDPIDRLRRANLACEDDKLMIYGLPWMTTQTSALSINSKPIVYKDCAKLLRSINGSQPV SLNDVLRRExpression Region
1-128aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1416Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
Anti-DYKDDDDK tag (L5) Affinity Gel
-tag fusion protein is in soluble form, this supernatant is ready for L5 column purification. If the DYKDDDDK-tag fusion protein is in an insoluble form, solubilize the inclusion body after centrifugation step (Step 6), refold the protein properly
A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
, see ref. 3 ). As a first step in the construction of a recombinant vaccinia virus, the gene encoding a protein in the form of a cDNA is cloned into a plasmid vector under the control of an early or late vaccinia virus promoter (4 –
by the Leporipoxvirus, Shope fibroma virus (SFV), can be coupled with SFV-promoted reactivation reactions to rapidly construct recombinant vaccinia viruses in high yields (25–100% recombinant progeny). The reactivated vaccinia viruses are easily purified free of the SFV
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