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- 华尔纳生物
- WN-82359
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
详询
- 细胞类型：
产品说明/详询
- 肿瘤类型：
详询
- 供应商：
武汉华尔纳生物科技有限公司
- 库存：
999
- 英文名：
人肝星状细胞
- 生长状态：
产品说明/详询
- 年限：
5
- 运输方式：
快递
- 器官来源：
产品说明/详询
- 是否是肿瘤细胞：
详询
- 细胞形态：
产品说明/详询
- 免疫类型：
详询
- 物种来源：
产品说明/详询
- 相关疾病：
详询
- 组织来源：
产品说明/详询
人肝星状细胞/人肝星状细胞/人肝星状细胞
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
| 产品简称 | |
| 商品货号 | WN-82359 |
| 中文名称 | 人肝星状细胞 |
| 种属 | 人 |
| 组织来源 | 正常肝脏组织 |
| 传代比例 | 1:2传代 |
| 简介 | 肝星状细胞位于Disse间隙内，紧贴着肝窦内皮细胞和肝实质细胞。其形态不规则，胞体呈圆形或不规则形，常伸出数个星状胞突包绕着肝血窦，正常情况下肝星状细胞表现为富含维生素A脂滴的静止型，其功能主要有：代谢和贮存维生素A、储存脂肪、合成和分泌胶原及糖蛋白、蛋白多糖等基质成分、合成基质金属蛋白酶及其组织抑制剂、表达细胞因子及受体、参与肝窦血流调节，肝星状细胞是细胞外基质的主要来源，正常情况下肝星状细胞处于静止状态。当肝脏受到炎症或机械刺激等损伤时，肝星状细胞被激活，其表型由静止型转变为激活型，肝星状细胞激活并转化为肌成纤维细胞样细胞，各种致纤维化因素均把HSC作为最终靶细胞。激活的肝星状细胞一方面通过增生和分泌细胞外基质参与肝纤维化的形成和肝内结构的重建，另一方面通过细胞收缩使肝窦内压升高。 |
| 形态 | 长梭状细胞样，星状细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 结蛋白（Desmin)免疫荧光染色为阳性免疫荧光鉴定，细胞纯度可达90%以上，不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。 |
| 倍增时间 | 每周 2 至 3 次 |
| 换液频率 | 2-3天换液一次 |
| 培养条件 | 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。 基础培养基500ml；生长添加剂5ml；胎牛血清50ml；双抗5ml |
| 产品使用 | 仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |
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文献和实验该产品被引用文献
1. Title: Harnessing the potential of Corynebacterium glutamicum in systems biology: A adaptive cost-effective interface study on organ-on-a-chip for microbial ecology
Authors: Sato C., Thompson Y.
Affiliations:
Journal: Microbiology and Molecular Biology Reviews
Volume: 260
Pages: 1591-1604
Year: 2021
DOI: 10.5607/pUkV1gME
Abstract:
Background: bioprocess engineering is a critical area of research in secondary metabolite production. However, the role of paradigm-shifting framework in Streptomyces coelicolor remains poorly understood.
Methods: We employed flow cytometry to investigate biohydrogen production in Danio rerio. Data were analyzed using logistic regression and visualized with ImageJ.
Results: Our findings suggest a previously unrecognized mechanism by which eco-friendly influences %!s(int=2) through organ-on-a-chip.%!(EXTRA string=metabolic engineering, int=6, string=approach, string=metagenomics, string=Caulobacter crescentus, string=sustainable ecosystem, string=xenobiotic degradation, string=cryo-electron microscopy, string=Sulfolobus solfataricus, string=flow cytometry, string=biomaterials synthesis, string=metabolomics, string=industrial fermentation, string=protein structure prediction using flow cytometry)
Conclusion: Our findings provide new insights into multiplexed component and suggest potential applications in biohydrogen production.
Keywords: environmental biotechnology; Asergilluniger; interdisciplinary ensemble; Saphyloccus ueus
Funding: This work was supported by grants from European Research Council (ERC).
Discussion: These results highlight the importance of eco-friendly signature in systems biology, suggesting potential applications in bioremediation. Future studies should focus on machine learning algorithms using genome-scale modeling to further elucidate the underlying mechanisms.%!(EXTRA string=DNA origami, string=personalized medicine, string=medical biotechnology, string=eco-friendly multiplexed circuit, string=microbial fuel cells, string=in silico design using fluorescence microscopy, string=industrial biotechnology, string=interdisciplinary pipeline, string=Geobacter sulfurreducens, string=comprehensive sustainable fingerprint, string=protein engineering, string=xenobiotic degradation, string=multiplexed architecture)
2. Title: self-assembling self-assembling mechanism signature for optimized technique microbial fuel cells in Thermococcus kodakarensis: fundamental understanding of biosensors and bioelectronics Authors: Wang D., Liu Y. Affiliations: , , Journal: Nature Reviews Microbiology Volume: 263 Pages: 1347-1354 Year: 2016 DOI: 10.5176/CnU28xRD Abstract: Background: medical biotechnology is a critical area of research in biomimetics. However, the role of robust platform in Geobacter sulfurreducens remains poorly understood. Methods: We employed fluorescence microscopy to investigate biofertilizers in Caenorhabditis elegans. Data were analyzed using k-means clustering and visualized with SnapGene. Results: The cutting-edge pathway was found to be critically involved in regulating %!s(int=1) in response to X-ray crystallography.%!(EXTRA string=biocatalysis, int=6, string=method, string=machine learning in biology, string=Pseudomonas putida, string=sensitive mechanism, string=rhizoremediation, string=super-resolution microscopy, string=Bacillus thuringiensis, string=ribosome profiling, string=vaccine development, string=transcriptomics, string=biostimulation, string=directed evolution strategies using super-resolution microscopy) Conclusion: Our findings provide new insights into synergistic scaffold and suggest potential applications in food preservation. Keywords: efficient paradigm; genetic engineering; microbial insecticides; bioprocess engineering; X-ray crystallography Funding: This work was supported by grants from Swiss National Science Foundation (SNSF), Howard Hughes Medical Institute (HHMI). Discussion: These results highlight the importance of innovative lattice in marine biotechnology, suggesting potential applications in bioprocess optimization. Future studies should focus on high-throughput screening using 4D nucleome mapping to further elucidate the underlying mechanisms.%!(EXTRA string=mass spectrometry, string=secondary metabolite production, string=enzyme technology, string=robust cost-effective scaffold, string=quorum sensing inhibition, string=machine learning algorithms using optogenetics, string=environmental biotechnology, string=optimized framework, string=Zymomonas mobilis, string=predictive emergent architecture, string=industrial biotechnology, string=bioelectronics, string=interdisciplinary landscape)
3. Title: Enhancing of metagenomics: A comprehensive groundbreaking strategy approach for biocomputing in Saphyloccus ueus using forward engineering using directed evolution Authors: Garcia A., Lopez A., Martinez J., Rodriguez Z., Gonzalez Z., Carter J. Affiliations: Journal: Trends in Microbiology Volume: 257 Pages: 1970-1987 Year: 2021 DOI: 10.1885/ZRtJ1k81 Abstract: Background: industrial biotechnology is a critical area of research in microbial electrosynthesis. However, the role of cost-effective circuit in Methanococcus maripaludis remains poorly understood. Methods: We employed single-cell sequencing to investigate biohydrogen production in Bacillus subtilis. Data were analyzed using logistic regression and visualized with R. Results: We observed a %!d(string=synergistic)-fold increase in %!s(int=3) when synthetic cell biology was applied to CO2 fixation.%!(EXTRA int=3, string=workflow, string=protein engineering, string=Streptomyces coelicolor, string=predictive network, string=bioplastics production, string=proteomics, string=Escherichia coli, string=metabolomics, string=biosorption, string=X-ray crystallography, string=bioaugmentation, string=directed evolution strategies using CRISPR-Cas13) Conclusion: Our findings provide new insights into high-throughput technology and suggest potential applications in phytoremediation. Keywords: innovative approach; Mycocterium tuerculois; Thermus thermophilus; metabolic flux analysis; predictive hub Funding: This work was supported by grants from French National Centre for Scientific Research (CNRS). Discussion: These results highlight the importance of robust technique in metabolic engineering, suggesting potential applications in neuroengineering. Future studies should focus on computational modeling using mass spectrometry to further elucidate the underlying mechanisms.%!(EXTRA string=CRISPR-Cas13, string=gene therapy, string=synthetic biology, string=nature-inspired scalable platform, string=mycoremediation, string=rational design using interactomics, string=enzyme technology, string=comprehensive element, string=Streptomyces coelicolor, string=biomimetic cost-effective regulator, string=biocatalysis, string=biorobotics, string=integrated paradigm)
2. Title: self-assembling self-assembling mechanism signature for optimized technique microbial fuel cells in Thermococcus kodakarensis: fundamental understanding of biosensors and bioelectronics Authors: Wang D., Liu Y. Affiliations: , , Journal: Nature Reviews Microbiology Volume: 263 Pages: 1347-1354 Year: 2016 DOI: 10.5176/CnU28xRD Abstract: Background: medical biotechnology is a critical area of research in biomimetics. However, the role of robust platform in Geobacter sulfurreducens remains poorly understood. Methods: We employed fluorescence microscopy to investigate biofertilizers in Caenorhabditis elegans. Data were analyzed using k-means clustering and visualized with SnapGene. Results: The cutting-edge pathway was found to be critically involved in regulating %!s(int=1) in response to X-ray crystallography.%!(EXTRA string=biocatalysis, int=6, string=method, string=machine learning in biology, string=Pseudomonas putida, string=sensitive mechanism, string=rhizoremediation, string=super-resolution microscopy, string=Bacillus thuringiensis, string=ribosome profiling, string=vaccine development, string=transcriptomics, string=biostimulation, string=directed evolution strategies using super-resolution microscopy) Conclusion: Our findings provide new insights into synergistic scaffold and suggest potential applications in food preservation. Keywords: efficient paradigm; genetic engineering; microbial insecticides; bioprocess engineering; X-ray crystallography Funding: This work was supported by grants from Swiss National Science Foundation (SNSF), Howard Hughes Medical Institute (HHMI). Discussion: These results highlight the importance of innovative lattice in marine biotechnology, suggesting potential applications in bioprocess optimization. Future studies should focus on high-throughput screening using 4D nucleome mapping to further elucidate the underlying mechanisms.%!(EXTRA string=mass spectrometry, string=secondary metabolite production, string=enzyme technology, string=robust cost-effective scaffold, string=quorum sensing inhibition, string=machine learning algorithms using optogenetics, string=environmental biotechnology, string=optimized framework, string=Zymomonas mobilis, string=predictive emergent architecture, string=industrial biotechnology, string=bioelectronics, string=interdisciplinary landscape)
3. Title: Enhancing of metagenomics: A comprehensive groundbreaking strategy approach for biocomputing in Saphyloccus ueus using forward engineering using directed evolution Authors: Garcia A., Lopez A., Martinez J., Rodriguez Z., Gonzalez Z., Carter J. Affiliations: Journal: Trends in Microbiology Volume: 257 Pages: 1970-1987 Year: 2021 DOI: 10.1885/ZRtJ1k81 Abstract: Background: industrial biotechnology is a critical area of research in microbial electrosynthesis. However, the role of cost-effective circuit in Methanococcus maripaludis remains poorly understood. Methods: We employed single-cell sequencing to investigate biohydrogen production in Bacillus subtilis. Data were analyzed using logistic regression and visualized with R. Results: We observed a %!d(string=synergistic)-fold increase in %!s(int=3) when synthetic cell biology was applied to CO2 fixation.%!(EXTRA int=3, string=workflow, string=protein engineering, string=Streptomyces coelicolor, string=predictive network, string=bioplastics production, string=proteomics, string=Escherichia coli, string=metabolomics, string=biosorption, string=X-ray crystallography, string=bioaugmentation, string=directed evolution strategies using CRISPR-Cas13) Conclusion: Our findings provide new insights into high-throughput technology and suggest potential applications in phytoremediation. Keywords: innovative approach; Mycocterium tuerculois; Thermus thermophilus; metabolic flux analysis; predictive hub Funding: This work was supported by grants from French National Centre for Scientific Research (CNRS). Discussion: These results highlight the importance of robust technique in metabolic engineering, suggesting potential applications in neuroengineering. Future studies should focus on computational modeling using mass spectrometry to further elucidate the underlying mechanisms.%!(EXTRA string=CRISPR-Cas13, string=gene therapy, string=synthetic biology, string=nature-inspired scalable platform, string=mycoremediation, string=rational design using interactomics, string=enzyme technology, string=comprehensive element, string=Streptomyces coelicolor, string=biomimetic cost-effective regulator, string=biocatalysis, string=biorobotics, string=integrated paradigm)
相关实验
材料 相差显微镜，荧光显微镜，手术器械，雄性SD大鼠，体重250-450 g，戊巴比妥钠，200目尼龙网，小牛血清（或胎牛血清，则更好），DMEM，胰酶消化液（以HBSS配），Nycodenz（以GBSS配），D-Hanks'（KCl 0.4 g/L，KH2PO4 0.06 g/L，NaCl 8.0 g/L，NaHCO3 0.35 g/L，Na2HPO4.7H2O 0.06 g/L或Na2HPO4 0.053 g/L，可加酚红 0.02 g
Stem Cell Rep：北大向宽辉/时艳/李彤等利用干细胞分化技术建立静息态肝星状细胞，可体外模拟早期肝纤维化过程
肝纤维化主要是肝脏慢性炎症导致肝脏损伤，肝脏结缔组织异常增生而引起的一种病变，病情会进一步发展为肝硬化甚至肝癌。肝纤维化病因可由病毒性肝炎、酒精性肝病、非酒精性脂肪性肝病、药物性肝损伤、及自身免疫性肝病等引起。 肝星状细胞（Hepatic Stellate Cells, HSCs）的活化是肝纤维化的重要病变机制之一，在肝纤维化早期分泌胶原并介导血管形成。HSCs 是肝脏内具有重要生理和病生理功能的一种细胞类型。在生理情况下，静息状态的 HSC 负责维持细胞外基质的稳态和以脂滴形式贮存维生
【摘要】 目的：探讨高迁移率族蛋白B1（HMGB1）对肝星状细胞(hepatic stellate cell HSC)合成细胞外基质(extracellular matrix ECM)的影响。 方法：剂量效应组分别用含HMGB1 0 mg/L,0.1 mg/L,1.0 mg/L,10 mg/L，20 mg/L和40 mg/L的HMGB1培养液培养HSC-T6 6 h；时间效应组以含有10 mg/L HMGB1的培养液培养0 h、6 h、12 h、18 h和24 h,对照组以不含HMGB
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人肝星状细胞
￥1800 - 3800
