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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
"-20°C/-80°C"
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名:
Customized Mouse Rnasek Protein (in vitro E.coli)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20ug
Alternative Name(s)
Ribonuclease kappa; RNase K; RNase kappa EC= 3.1.-.-Editorial/Sponsord
EditorialUniprot ID
Q8K3C0Gene Names
RnasekOrganism
MouseAASequence
MASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYN LYEQVSYNCFIAAGLYLLLGGFSFCQVRLNKRKEYMVRExpression Region
1-98aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1378Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动,请以官网信息为准
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文献和实验**产品信息可能有变动,请以官网信息为准
Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
RNase Protection Assay (Bowtell Lab)
1. Cut 10ug of plasmid DNA where you want the transcript to end. 2. Add an equal volume of 2X PK buffer: 200mM TrisHCl pH 7.5 25mM EDTA 300mM NaCl 2% SDS 200ug/ml proteinase K 3. 37� for 30min and then extract with phenol
K cocktail (per 20 samples): 390 µl Proteinase K buffer 30 µl Proteinase K 30 µl yeast tRNA Mix and add 18 µl aliquots of the cocktail to new Eppendorf tubes. 16.Using a pipettor, extract the RNase digests from underneath the oil (try to avoid
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