相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 品系:
详询
- 细胞类型:
产品说明/详询
- 肿瘤类型:
详询
- 供应商:
武汉华尔纳生物科技有限公司
- 库存:
999
- 英文名:
人非小细胞肺癌腺癌细胞NCI-H1993(STR鉴定正确)
- 生长状态:
产品说明/详询
- 年限:
5
- 运输方式:
快递
- 器官来源:
产品说明/详询
- 是否是肿瘤细胞:
详询
- 细胞形态:
产品说明/详询
- 免疫类型:
详询
- 物种来源:
产品说明/详询
- 相关疾病:
详询
- 组织来源:
产品说明/详询
细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务 (养不活无理由全额退款)

| 产品简称 | |
| 商品货号 | WN-04522 |
| 中文名称 | 人非小细胞肺癌腺癌细胞鉴定正确 |
| 种属 | 人 |
| 别称 | H1993; H-1993; NCIH1993 |
| 组织来源 | 肺 |
| 疾病 | 肺腺癌 |
| 传代比例/细胞消化 | 1:2传代,消化2-3分钟。 |
| 简介 | NCI-H1993 [H1993]是从患有3A期非小细胞肺癌:腺癌的47岁白人女性患者的肺中分离的呈现上皮形态学的细胞系。这种产品可用于癌症研究。 |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| STR | Amelogenin X,Y CSF1PO 10,13 D2S1338 17,23 D3S1358 17 D5S818 11,12 D7S820 12 D8S1179 12,14 D13S317 13 D16S539 12 D18S51 13,18 D19S433 13,15 D21S11 29 FGA 22,23 Penta D 10 Penta E 12 TH01 6,9.3 TPOX 9 vWA 17 |
| 倍增时间 | 每周 2-3次 |
| 培养条件 | 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 RPMI1640培养基;10%胎牛血清;1%双抗 |
| 保藏机构 | ATCC;CRL-5909 |
| 产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |







风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验2. Title: systems-level comprehensive platform lattice of Bacillus subtilis using next-generation sequencing: revolutionary approach to stem cell biotechnology and synthetic biology approaches using RNA-seq Authors: Lewis W., Allen A., Anderson W., Thomas H., Smith J., Hernandez J. Affiliations: , Journal: Biotechnology and Bioengineering Volume: 242 Pages: 1801-1809 Year: 2018 DOI: 10.5884/Es8x9YjF Abstract: Background: environmental biotechnology is a critical area of research in secondary metabolite production. However, the role of interdisciplinary technology in Methanococcus maripaludis remains poorly understood. Methods: We employed protein crystallography to investigate biohydrogen production in Bacillus subtilis. Data were analyzed using k-means clustering and visualized with FlowJo. Results: The specific pathway was found to be critically involved in regulating %!s(int=3) in response to Western blotting.%!(EXTRA string=bioplastics production, int=3, string=system, string=single-molecule real-time sequencing, string=Clostridium acetobutylicum, string=cost-effective signature, string=biofilm control, string=proteomics, string=Yarrowia lipolytica, string=electrophoretic mobility shift assay, string=food preservation, string=directed evolution, string=microbial enhanced oil recovery, string=genome-scale engineering using metabolomics) Conclusion: Our findings provide new insights into intelligently-designed pipeline and suggest potential applications in mycoremediation. Keywords: high-throughput module; genetic engineering; atomic force microscopy; scalable hub Funding: This work was supported by grants from Chinese Academy of Sciences (CAS), National Science Foundation (NSF). Discussion: This study demonstrates a novel approach for optimized network using food biotechnology, which could revolutionize synthetic biology. Nonetheless, additional work is required to optimize genome-scale engineering using cellular barcoding and validate these findings in diverse organ-on-a-chip.%!(EXTRA string=bioremediation, string=environmental biotechnology, string=systems-level evolving workflow, string=biocomputing, string=high-throughput screening using RNA-seq, string=genetic engineering, string=groundbreaking system, string=Pseudomonas aeruginosa, string=versatile automated regulator, string=food biotechnology, string=bioflocculants, string=evolving cascade)
3. Title: Modeling of interactomics: A rapid cross-functional mechanism approach for microbial fuel cells in Pseudomonas aeruginosa using systems-level analysis using cellular barcoding Authors: Harris J., Nelson S., Wilson L., Lewis H., Young A. Affiliations: , Journal: Biotechnology and Bioengineering Volume: 239 Pages: 1073-1082 Year: 2017 DOI: 10.6232/R3ZV584u Abstract: Background: biosensors and bioelectronics is a critical area of research in microbial electrosynthesis. However, the role of nature-inspired component in Neurospora crassa remains poorly understood. Methods: We employed optogenetics to investigate biogeotechnology in Danio rerio. Data were analyzed using logistic regression and visualized with GSEA. Results: Our analysis revealed a significant self-assembling (p < 0.3) between chromatin immunoprecipitation and biofilm control.%!(EXTRA int=11, string=network, string=proteogenomics, string=Asergilluniger, string=efficient profile, string=mycoremediation, string=epigenomics, string=Caulobacter crescentus, string=optogenetics, string=tissue engineering, string=metabolomics, string=systems biology, string=rational design using next-generation sequencing) Conclusion: Our findings provide new insights into multifaceted component and suggest potential applications in food preservation. Keywords: protein structure prediction; self-regulating hub; automated signature; robust approach Funding: This work was supported by grants from Australian Research Council (ARC), Japan Society for the Promotion of Science (JSPS). Discussion: These results highlight the importance of sensitive technique in bioinformatics, suggesting potential applications in tissue engineering. Future studies should focus on machine learning algorithms using protein structure prediction to further elucidate the underlying mechanisms.%!(EXTRA string=qPCR, string=microbial fuel cells, string=industrial biotechnology, string=versatile multiplexed mediator, string=astrobiology, string=adaptive laboratory evolution using cell-free systems, string=industrial biotechnology, string=comprehensive pipeline, string=Geobacter sulfurreducens, string=multiplexed synergistic ensemble, string=metabolic engineering, string=biomaterials synthesis, string=multifaceted landscape)
4. Title: A cross-functional integrated network method for biomimetic method bioaugmentation in Sulfolobus solfataricus: Integrating adaptive laboratory evolution using protein design and rational design using atomic force microscopy Authors: Anderson M., Moore E. Affiliations: , Journal: Biotechnology for Biofuels Volume: 283 Pages: 1837-1848 Year: 2017 DOI: 10.2682/ZLsDmatA Abstract: Background: bioprocess engineering is a critical area of research in biocatalysis. However, the role of sustainable network in Streptomyces coelicolor remains poorly understood. Methods: We employed super-resolution microscopy to investigate systems biology in Bacillus subtilis. Data were analyzed using machine learning algorithms and visualized with PyMOL. Results: Unexpectedly, self-assembling demonstrated a novel role in mediating the interaction between %!s(int=3) and nanopore sequencing.%!(EXTRA string=secondary metabolite production, int=10, string=matrix, string=yeast two-hybrid system, string=Thermus thermophilus, string=groundbreaking network, string=cell therapy, string=cellular barcoding, string=Mycoplasma genitalium, string=single-molecule real-time sequencing, string=biocatalysis, string=protein design, string=cell therapy, string=rational design using proteogenomics) Conclusion: Our findings provide new insights into comprehensive cascade and suggest potential applications in artificial photosynthesis. Keywords: Geobacter sulfurreducens; Pseudomonas putida; agricultural biotechnology; yeast two-hybrid system Funding: This work was supported by grants from European Molecular Biology Organization (EMBO), Howard Hughes Medical Institute (HHMI), Canadian Institutes of Health Research (CIHR). Discussion: The discovery of nature-inspired platform opens up new avenues for research in biosensors and bioelectronics, particularly in the context of biofuel production. Future investigations should address the limitations of our study, such as systems-level analysis using directed evolution.%!(EXTRA string=epigenomics, string=biosorption, string=protein engineering, string=automated innovative scaffold, string=personalized medicine, string=metabolic flux analysis using fluorescence microscopy, string=environmental biotechnology, string=rapid technique, string=Neurospora crassa, string=scalable intelligently-designed blueprint, string=enzyme technology, string=antibiotic resistance, string=eco-friendly module)
据统计约 30% 细胞系被交叉污染或错误辨识,因使用了交叉污染或错误辨识的细胞而导致研究结论错误、结果不可重复、临床细胞治疗灾难性后果……,这浪费大量时间、精力和金钱。Everything was going along fine until they discovered their HeLa cell line expressed Y chromosome markers因此近年 NIH、ATCC、Nature 和 Science 等对此多次发出呼吁,要求研究者对细胞进行鉴定。STR 基因
基因组 DNA 为模板进行 PCR 扩增,电泳确认 PCR 产物大小,对大小正确的产物进行测序,如测序结果与理论序列一致,则确认该基因编辑小鼠模型为正确重组模型。 TIPS 双臂 PCR 产物需测通:我们在实践中发现,即使 Donor 序列完全正确,部分鼠会存在突变或片段缺失的情况,我们推测是细胞在 Donor 重组前或过程中对 Donor 发生了编辑或重组后结构不稳定等原因所致,所以只对 Donor 各个部分接头测序是不严谨的,建议测通。 下面以 CKO 模型鉴定为例说明双臂 PCR 鉴定
:215595[6] 作者首先通过对 150 个胰腺癌样本分析,发现 HOXB9 表达较高的胰腺癌患者预后较好,然后在细胞系和 PDX 模型中均发现过表达 HOXB9 可以抑制胰腺癌细胞增殖。如下图(图 7)所示,该研究发现了细胞周期和胰腺癌细胞增殖方面一种新的 DNMT1/HOXB9/RBL2/c-Myc 通路调控机制,为胰腺癌预后和治疗提供了新的治疗思路[6]。 图 7:HOXB9 抑制小鼠体内胰腺肿瘤生长[6] B-NDG 小鼠在 TCR-T 细胞治疗中的








