多聚左旋赖氨酸 *Sigma货号：P8920-500ML/P

应用
Poly-L-lysine solution has been used to attach endothelial cell cross-sections onto UV crosslinked PET film frame slides. [1] Poly-L-lysine coated glass slides are used to place tissue sections for immunohistochemical detection. [2] Poly-L-lysine polymers can be used to promote cell adhesion to solid substrates, bind with methotrexate to increase drug transport, microencapsulation of pancreatic islets, cell microencapsulation techniques, microarray slide coatings, and chromosome preparations. Poly-L-lysine with lower molecular weight (30000-70000) has lower viscosity in solution, but polylysine with higher molecular weight provides more attachment sites in each molecule

生化/生理作用

聚-L-赖氨酸是一种非特异性的细胞附着因子，通过增强细胞膜负离子与培养基表面的静电相互作用，促进细胞对固体基质的粘附
当其吸附到细胞培养表面时，聚-L-赖氨酸的作用是增加可用于细胞结合的带正电荷位点的数量
对于可降解聚-L-赖氨酸的细胞，应采用聚-D-赖氨酸作为附着因子
组分 聚-L-赖氨酸是一种带正电荷的氨基酸聚合物，每个赖氨酸残基约有一个HBr
氢溴suan盐可使聚-L-赖氨酸以可溶于水的结晶形式存在
在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键