聚-D-赖氨酸氢溴suan盐 *Sigma货号：P7280-

一般描述

多聚-D-赖氨酸氢溴suan盐（PDL）是一种由赖氨酸组成的带正电的聚合物，其中的每个赖氨酸单元结合一个氢溴suan分子
这种氢溴suan分子赋予多聚-D-赖氨酸独特的特性，令其形成晶体并易溶于水基溶液
应用
多聚-D-赖氨酸氢溴suan盐涂层已用于：
在原代神经元培养物的免疫荧光显微镜检查中，包被组织培养板孔和盖玻片，‘以研究与寨卡病毒感染有关的神经功能失调
[1] 包被盖玻片以增强大鼠肠道分离的肠肌层细胞的附着和增殖
[2] 处理载玻片的表面以实现细胞附着，以研究大鼠小肠的肠肌层丛（myenteric plexus ）
[3] 聚-D-赖氨酸聚合物可用于制备细胞附着的表面
D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆
较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点

生化/生理作用

Poly-L-lysine (PDL) hydrobromide salt is a non-specific adhesion factor for cells, which promotes adhesion between cells and solid matrix by enhancing the electrostatic interaction between negatively charged ions on the cell membrane surface and the culture surface. After being absorbed by the surface of cell culture, poly-D-lysine can increase the number of positively charged cell binding sites. Characteristics and advantages Sterilization by gamma radiation. Components Poly-D-lysine is a positively charged amino acid polymer, in which each lysine residue has approximately one HBr. Hydrogen bromide salt can make poly-D-lysine in a water-soluble crystalline form. A small amount of products may be found in the β structure, as HBr interferes with hydrogen bonds between amino and carboxyl groups or N - or O-containing moieties.