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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant BKPyV LT-AG/Large T antigen Protein, N-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1280.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1880.0 |
| 规格: | 1mg | 产品价格: | ¥9776.0 |
| 产品名 | Recombinant BKPyV LT-AG/Large T antigen Protein, N-His |
| 货号 | YVV36701 |
| 别名 | Large T antigen, LT, LT-AG, 3.6.4.- |
| 表达系统 | E. coli |
| Accession号 | P14999 |
| 种属 | BK polyomavirus (strain AS) (BKPyV) |
| 蛋白长度 | Lys133-Asp629 |
| 预测分子量 | 60.06 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验chaperone complexes of large HSPs and protein antigen may be exploited for augmentation of an antigen-specific immune response. The methods for the preparation of the recombinant protein antigen chaperone complex and characterization of its T-cell priming
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
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