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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant flavus Uricase/Uox Protein, C-hFc (IgG1)
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug
| Expression system | Mammalian Cells |
| Accession | Q00511 |
| Protein length | Met1-Lys302 |
| Alternative Names | Uricase, 1.7.3.3, Rasburicase, Urate oxidase, uaZ, Uox |
| Form | Lyophilized |
| Predicted molecular weight | 62.45 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | flavus |
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文献和实验Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
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