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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human MDK/Midkine Protein, N-His-SUMO
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug
| Expression system | E. coli |
| Accession | P06802 |
| Protein length | Lys617-Asp906 |
| Alternative Names | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E- 1, Lymphocyte antigen 41, Ly-41, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, 3.1.4.1, Nucleotide pyrophosphatase, ase, 3.6.1.9, Nucleotide diphosphatase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form, E1, s, Pc1, Pdnp1 |
| Form | Lyophilized |
| Predicted molecular weight | 36.29 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | Mus musculus (Mouse) |
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文献和实验Purification of E1 and E1-Like Enzymes
that has been adapted for the isolation of E1 paralogs. We describe the facile purification of active E1 from outdated human red blood cells in yields (2–4 nmol/U of blood) that make this an attractive alternative to expression of the proteolytically labile recombinant
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
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