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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
abinscience
- 检测范围:
1.56 - 100 ng/mL
- 检测方法:
Colorimetric
- 应用:
ELISA
- 适应物种:
Not Species Specific
- 标记物:
Unconjugated
- 样本:
Plasma, Serum
- 灵敏度:
0.65 ng/mL
- 规格:
96T

| Product name | HMPV N/Nucleoprotein ELISA Kit |
|---|---|
| Catalog No. | VK571018 |
| Applications | ELISA |
| Accession | \ |
| Stability and Storage | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. |
| Detection method | Colorimetric |
| Sample type | Plasma, Serum |
| Assay type | Quantitative |
| Sensitivity | 0.65 ng/mL |
| Background | \ |
| Target | N/Nucleoprotein |
| Range | 1.56 - 100 ng/mL |
| Recovery | 80-120% |
| Alternative Names | \ |
| Clone ID | \ |
| Shipping | 2-8 ℃ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Kolosova A Y, Shim W B, Yang Z Y, et al. Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B 1. Stabilization of ELISA kit components and application to grain samples[J]. Analytical and bioanalytical chemistry, 2006, 384: 286-411.
(positive),而是病人(patient)的缩写,不应误解。为避免混淆,更宜用 S/N 表示。在早期的间接法 ELISA 中,有些作者定出 S/N 为阳性标准,现多为各种测定所沿用。实际上每一测定系统应该用实验求出各自的 S/N 的阈值。更应注意的是,N 所代表的阴性对照是不含受检物质的人血清。有的试剂盒中所设阴性对照为不含蛋白质或蛋白质含量较底的缓冲液,以致反应后产生的本底可能较正常人血清的本底低得多。因此,这类试剂盒规,如 N (2) 竞争法 在竞争法 ELISA 中,阴性孔呈色深于阳性孔。阴性
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
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