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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
abinscience
- 检测范围:
0.07 - 50 ng/mL
- 检测方法:
Colorimetric
- 应用:
ELISA
- 适应物种:
Not Species Specific
- 标记物:
Unconjugated
- 样本:
Plasma, Serum
- 灵敏度:
0.15 ng/mL
- 规格:
96T

| Product name | MARV Nucleoprotein (NP) ELISA Kit |
|---|---|
| Catalog No. | VK660018 |
| Applications | ELISA |
| Accession | \ |
| Stability and Storage | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. |
| Detection method | Colorimetric |
| Sample type | Plasma, Serum |
| Assay type | Quantitative |
| Sensitivity | 0.15 ng/mL |
| Background | \ |
| Target | NP/Nucleoprotein |
| Range | 0.07 - 50 ng/mL |
| Recovery | 80-120% |
| Alternative Names | \ |
| Clone ID | \ |
| Shipping | 2-8 ℃ |
| Note | For Research Use Only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Kolosova A Y, Shim W B, Yang Z Y, et al. Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B 1. Stabilization of ELISA kit components and application to grain samples[J]. Analytical and bioanalytical chemistry, 2006, 384: 286-389.
除>95%的蛋白提取和样品制备过程中所使用的去垢剂,而蛋白和多肽损失极少。此树脂适用于常用去垢剂的去除,如浓度在0.5-1%的SDS、Triton X-100、NP-40和CHAPS。目前有预装的离心柱和离心板形式,最多可处理100 µL样品。另外,您也可以购买HiPPR Detergent Removal Spin Column Kit,其中包含树脂浆和空的离心柱,便于您自己调整样品体积。 整个处理过程相当简单,仅需15分钟。首先是离心去除保存液,之后用缓冲液平衡树脂并离心,重复
重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂,如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇(DTT)、β-巯基乙醇等反复多次进行,因去垢剂洗涤能力随溶液离子强度升高而加强,在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上,而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜
纯的包涵体 对于包涵体的纯化,包涵体的前处理是很重要的。 包涵体中主要含有重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂,如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇(DTT)、β-巯基乙醇等反复多次进行,因去垢剂洗涤能力随溶液离子强度升高而加强,在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上,而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原
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